John Y. Anagli, Ph.D.

Biography

• B.S., M.S., Chemical Sciences, Universite Catholique de Louvain, Louvain-La-Neuve, Belgium 1981-1986
• Postgraduate, Medical & Pharmaceutical Research, Vrije Universiteit Brussel School of Medicine, Brussels, Belgium 1986-1987
• M.S., Ph.D., Biochemistry, Universität Basel, Basel, Switzerland 1987-1991
• Protease Inhibitor Design, Friedrich Miescher Institute for Biomedical Research/Norvatis Ltd., Basel, Switzerland 1991-1992

Awards, Honors and Publications

• 1998- 2001 Member and Faculty Participant, University of Michigan Multipurpose Arthritis and Musculoskeletal Diseases Center, Ann Arbor, MI
• 2000- Present Member, Proteases and Cancer Program, Karmanos Cancer Institute, School of Medicine, Wayne State University, Detroit, MI
• 2000- Present Member, Managing Editorial Board, Frontiers in Biosciences
• 2002- Present Member, University of Michigan Rheumatic Diseases Core Center, Ann Arbor, MI
• 2003- Present Member, Scientific Advisory Board, Department of Defense Center of Excellence for Breast Cancer Research, School of Medicine, Wayne State University, Detroit, MI

• 1987 FEBS Award: Advanced Course in Inorganic Biochemistry, Belgium
• 1988-1991 Friedrich Miescher Graduate Fellowship, Switzerland

• Anagli, J, Hagmann J, Shaw E. Investigation of the Role of Calpain as a Stimulus-Response Mediator in Human Platelets Using New Synthetic Inhibitors. Biochem J 1991;274:497-502. PMCID: PMC 1150166
• Angliker H, Anagli J, Shaw E. Inactivation of Calpain by Peptidyl Fluoro-methylketones. J Med Chem 1992;35:216-220. PMID:1732539
• Anagli J, Hagmann J, Shaw E. Affinity Labelling of the Ca2+-activated Neutral Proteinase in Intact Human Platelets. Biochem J 1993;289:93-99. PMCID:PMC 1132135
• Wikstrom P, Anagli J, Angliker H, Shaw E. Additional Peptidyl Diazomethyl Ketones Including Biotinylated Derivatives, Which Affinity-label Calpain and Related Cysteinyl Proteinases. J Enzyme Inhib 1993;6:259-269. PMID:1284963
• Hofmann F, Anagli J, Carafoli E, Vorherr T. Phosphorylation of the Calmodulin Binding Domain of the Plasma Membrane Ca2+ Pump by Protein Kinase C Reduces Its Interaction with Calmodulin and with Its Pump Receptor Site. J Biol Chem 1994;269:24298-24303. PMID:7929086
• Molinari M, Anagli J, Carafoli E. Ca2+ -activated Neutral Protease Is Active in the Erythrocyte Membrane in Its Nonautolyzed 80-kDa Form. J Biol Chem 1994;269:27992-27995. PMID:7961733
• Anagli J, Hofmann F, Quadroni M, Vorherr T, Carafoli E. The Calmodulin-binding Domain of the Inducible (Macrophage) Nitric Oxide Synthase Eur J Biochem 1995;233, 701-708. PMID:851832
• Molinari M, Anagli J, Carafoli E. PEST Sequences Do Not Influence Substrate Susceptibility to Calpain Proteolysis. J Biol Chem 1995;270:2032-2035. PMID:7836429
• Maslennikov IV, Sobol AG, Anagli J, James P, Vorherr T, Arseniev AS, Carafoli E. The Secondary Structure of Phospholamban: A Two-Dimensional NMR Study. Biochem Biophys Res Commun 1995;217:1200-1207. PMID:8554577
• Anagli J, Vilei EM, Molinari M, Calderara S, Carafoli E. Purification of Active Calpain by Peptide Affinity Chromatography on an Immobilized Peptide Inhibitor. Eur J Biochem 1996;241:948-954. PMID:8944787
• Fischer R, Wei Y, Anagli J, Berchtold MW. Calmodulin Binds to and Inhibits GTP Binding of the Ras-like GTPase Kir/Gem J Biol Chem 1996;271:25067-25070. PMID:8810259
• Vilei EM, Calderara S, Anagli J, Berardi S, Hitomi K, Maki M, Carafoli E. Functional Properties of Recombinant Calpain I and of Mutants Lacking Domains III and IV of the Catalytic Subunit J Biol Chem 1997; 272:25802-25808. PMID:9325309
• Seyfried DM, Veyna R, Han Y, Li K, Tang N, Betts RL, Weinsheimer S, Chopp M, Anagli J. A selective cysteine protease inhibitor is non-toxic and cerebroprotective in rats undergoing transient middle cerebral artery ischemia. Brain Res 2001;901:94-101. PMID:11368955
• Betts R, Weinsheimer S, Blouse G, Anagli, J. Structural Determinants of the Calpain Inhibitory Activity of Calpastatin Peptide B27-WT. J Biol Chem 2003;278:7800-7809. PMID:12500971
• Upadhya GA, Topp SA, Hotchkiss RS, Anagli J, Strasberg SM. Effect of Cold Preservation on Intracellular Calcium and Calpain Activity in Rat Sinusoidal Endothelial Cells. Hepatology 2003;37:313-323. PMID:12540781
• Betts R, Anagli J. The ß- and ?-CH2 of B27-WT's Leu11 and Ile18 side chains play a direct role in calpain inhibition. Biochemistry 2004;43:2596-2604. PMID:14992597
• Komissarov A.A, Andreasen PA, Bodker JS, Declerck PJ, Anagli JY, Shore JD. Additivity in effects of vitronectin and monoclonal antibodies against alpha-helix F of plasminogen activator inhibitor-1 on its reactions with target proteinases. J Biol Chem 2005;280:1482-9. PMID:15516335
• Yin, S, Lockett J, Meng Y, Biliran H, Blouse GE, Li X, Reddy N, Zhao Z, Lin X, Anagli J, Cher ML, Sheng S. Maspin retards cell detachment via a novel interaction with the uPA/uPAR system. Can Res 2006;66: 4173-81. PMID:16618739
• Anagli J, Abounit K, Stemmer P, Han Y, Allred L, Weinsheimer S, Movsisyan A, Seyfried D. Effects of cathepsin B and L inhibition on postischemic protein alterations in the brain. Biochem Biophys Res Comm 2008;366:86-91. PMID:18060871